Presented by Owen Griffin, Senior Director, R&D Microbiology
Watch on demand to learn how a new single medium, Rapid Sterility Medium (RSM), performs as well as or better for the growth promotion of test microorganisms compared to compendial sterility test media.
While both trypticase soy broth (TSB) and fluid thioglycollate medium (FTM) have served well as the growth media for sterility testing, the possibility of using other media has been examined in the light of the development of new, rapid microbiological test methods (RMM). The use of an alternative media, Rapid Sterility Medium (RSM) was investigated for its ability to replace both FTM and TSB. RSM is a custom non-selective medium that supports growth of a broad range of aerobic and anaerobic microorganisms.
Test data demonstrates the growth promotion performance of the media with direct inoculation of microorganisms and compares the time taken to detect growth compared to the compendial media. Overall, the RSM medium used in the direct inoculation mode is equivalent to the compendial media in its ability to grow all the test microorganisms used in this study <14 days. The results show growth with 90% of the microorganisms detected in <5 days for both the single RSM and the compendial TSB/FTM media and 100% of the microorganisms were detected within 10 days.